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10.1073/pnas.92.20.9176 Subramanian L, Crabb JW, Cox J, et al.: Ca2+ binding to EF hands 1 and 3 is essential for the interaction of apoptosis-linked gene-2 with Alix/AIP1 in ocular melanoma

Cellulose sponge is an adequate immobilization carrier for -EC production

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That means your concentration is higher than calculated

The results revealed a significant increase in viral proliferation in A549 kd-FcRn cells compared to wild-type (WT) cells, with a greater viral titer (Fig